Cocoa-based media for culturing Phytophthora palmivora (Butl.) Butl., causal agent of black pod disease of cocoa.
Identifieur interne : 002638 ( Main/Exploration ); précédent : 002637; suivant : 002639Cocoa-based media for culturing Phytophthora palmivora (Butl.) Butl., causal agent of black pod disease of cocoa.
Auteurs : R T Awuah [Ghana] ; M. FrimpongSource :
- Mycopathologia [ 0301-486X ] ; 2002.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Cacaoyer.
- croissance et développement : Phytophthora.
- isolement et purification : Phytophthora.
- microbiologie : Cacaoyer, Maladies des plantes.
- métabolisme : Agar-agar.
- Biomasse, Concentration en ions d'hydrogène, Milieux de culture.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Agar.
- chemistry : Cacao.
- growth & development : Phytophthora.
- isolation & purification : Phytophthora.
- microbiology : Cacao, Plant Diseases.
- Biomass, Culture Media, Hydrogen-Ion Concentration.
Abstract
Green cocoa pod husk agar (GCPA), ripe cocoa pod husk agar (RCPA), green cocoa bean agar (GCBA), ripe cocoa bean agar (RCBA), green cocoa mucilage agar (GCMA) and ripe cocoa mucilage agar (RCMA) were prepared and assessd for their clarity and for potential to support mycelial growth and sporulation of P. palmivora. Oatmeal agar (OMA), potato-dextrose agar (PDA), vegetable 8 juice agar (V8JA) and pineapple crown agar (PCA) were included for comparison. The highest radial growth rates of 8.3 and 7.2 mm/day were recorded, respectively, on OMA and GCPA but these were not significantly different (P < 0.05) from each other. The two media also supported good aerial mycelial growth but were not clear. Radial mycelial growth rates of 6.5, 7.0 and 6.6 mm/day were obtained on GCMA, RCPA and V8JA, respectively, and these rates were also not significantly different from each other. Of the three media, only the GCMA was clear and supported the best aerial mycelial growth. In comparison, the RCMA supported a significantly lower radial growth (4.6 mm/day) of P. palmivora than the three media. Growth rates were least on RCBA, PCA and PDA but sporulation was poorest on PDA, PCA and V8JA. GCMA was found to be the best medium based on all the growth parameters and media characteristics. GCMA has been used effectively to isolate/detect P. palmivora from infected cocoa pod tissues. Apart from differences in radial growth rate, both the GCMA and RCMA were similar in all other respects and are recommended for culturing P. palmivora.
DOI: 10.1023/a:1020415109308
PubMed: 12617500
Affiliations:
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Frimpong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12617500</idno><idno type="pmid">12617500</idno><idno type="doi">10.1023/a:1020415109308</idno><idno type="wicri:Area/Main/Corpus">002562</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002562</idno><idno type="wicri:Area/Main/Curation">002562</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002562</idno><idno type="wicri:Area/Main/Exploration">002562</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cocoa-based media for culturing Phytophthora palmivora (Butl.) Butl., causal agent of black pod disease of cocoa.</title><author><name sortKey="Awuah, R T" sort="Awuah, R T" uniqKey="Awuah R" first="R T" last="Awuah">R T Awuah</name><affiliation wicri:level="1"><nlm:affiliation>Department of Crop Science, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.</nlm:affiliation><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Crop Science, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi</wicri:regionArea><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Frimpong, M" sort="Frimpong, M" uniqKey="Frimpong M" first="M" last="Frimpong">M. Frimpong</name></author></analytic><series><title level="j">Mycopathologia</title><idno type="ISSN">0301-486X</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agar (metabolism)</term><term>Biomass (MeSH)</term><term>Cacao (chemistry)</term><term>Cacao (microbiology)</term><term>Culture Media (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Phytophthora (growth & development)</term><term>Phytophthora (isolation & purification)</term><term>Plant Diseases (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agar-agar (métabolisme)</term><term>Biomasse (MeSH)</term><term>Cacaoyer (composition chimique)</term><term>Cacaoyer (microbiologie)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Milieux de culture (MeSH)</term><term>Phytophthora (croissance et développement)</term><term>Phytophthora (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Agar</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cacao</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cacaoyer</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Cacaoyer</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Cacao</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agar-agar</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Culture Media</term><term>Hydrogen-Ion Concentration</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Concentration en ions d'hydrogène</term><term>Milieux de culture</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Green cocoa pod husk agar (GCPA), ripe cocoa pod husk agar (RCPA), green cocoa bean agar (GCBA), ripe cocoa bean agar (RCBA), green cocoa mucilage agar (GCMA) and ripe cocoa mucilage agar (RCMA) were prepared and assessd for their clarity and for potential to support mycelial growth and sporulation of P. palmivora. Oatmeal agar (OMA), potato-dextrose agar (PDA), vegetable 8 juice agar (V8JA) and pineapple crown agar (PCA) were included for comparison. The highest radial growth rates of 8.3 and 7.2 mm/day were recorded, respectively, on OMA and GCPA but these were not significantly different (P < 0.05) from each other. The two media also supported good aerial mycelial growth but were not clear. Radial mycelial growth rates of 6.5, 7.0 and 6.6 mm/day were obtained on GCMA, RCPA and V8JA, respectively, and these rates were also not significantly different from each other. Of the three media, only the GCMA was clear and supported the best aerial mycelial growth. In comparison, the RCMA supported a significantly lower radial growth (4.6 mm/day) of P. palmivora than the three media. Growth rates were least on RCBA, PCA and PDA but sporulation was poorest on PDA, PCA and V8JA. GCMA was found to be the best medium based on all the growth parameters and media characteristics. GCMA has been used effectively to isolate/detect P. palmivora from infected cocoa pod tissues. Apart from differences in radial growth rate, both the GCMA and RCMA were similar in all other respects and are recommended for culturing P. palmivora.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12617500</PMID><DateCompleted><Year>2003</Year><Month>03</Month><Day>26</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0301-486X</ISSN><JournalIssue CitedMedium="Print"><Volume>155</Volume><Issue>3</Issue><PubDate><Year>2002</Year></PubDate></JournalIssue><Title>Mycopathologia</Title><ISOAbbreviation>Mycopathologia</ISOAbbreviation></Journal><ArticleTitle>Cocoa-based media for culturing Phytophthora palmivora (Butl.) Butl., causal agent of black pod disease of cocoa.</ArticleTitle><Pagination><MedlinePgn>143-7</MedlinePgn></Pagination><Abstract><AbstractText>Green cocoa pod husk agar (GCPA), ripe cocoa pod husk agar (RCPA), green cocoa bean agar (GCBA), ripe cocoa bean agar (RCBA), green cocoa mucilage agar (GCMA) and ripe cocoa mucilage agar (RCMA) were prepared and assessd for their clarity and for potential to support mycelial growth and sporulation of P. palmivora. Oatmeal agar (OMA), potato-dextrose agar (PDA), vegetable 8 juice agar (V8JA) and pineapple crown agar (PCA) were included for comparison. The highest radial growth rates of 8.3 and 7.2 mm/day were recorded, respectively, on OMA and GCPA but these were not significantly different (P < 0.05) from each other. The two media also supported good aerial mycelial growth but were not clear. Radial mycelial growth rates of 6.5, 7.0 and 6.6 mm/day were obtained on GCMA, RCPA and V8JA, respectively, and these rates were also not significantly different from each other. Of the three media, only the GCMA was clear and supported the best aerial mycelial growth. In comparison, the RCMA supported a significantly lower radial growth (4.6 mm/day) of P. palmivora than the three media. Growth rates were least on RCBA, PCA and PDA but sporulation was poorest on PDA, PCA and V8JA. GCMA was found to be the best medium based on all the growth parameters and media characteristics. GCMA has been used effectively to isolate/detect P. palmivora from infected cocoa pod tissues. Apart from differences in radial growth rate, both the GCMA and RCMA were similar in all other respects and are recommended for culturing P. palmivora.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Awuah</LastName><ForeName>R T</ForeName><Initials>RT</Initials><AffiliationInfo><Affiliation>Department of Crop Science, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Frimpong</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Mycopathologia</MedlineTA><NlmUniqueID>7505689</NlmUniqueID><ISSNLinking>0301-486X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>9002-18-0</RegistryNumber><NameOfSubstance UI="D000362">Agar</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000362" MajorTopicYN="N">Agar</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002099" MajorTopicYN="N">Cacao</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>3</Month><Day>6</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>3</Month><Day>27</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>3</Month><Day>6</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12617500</ArticleId><ArticleId IdType="doi">10.1023/a:1020415109308</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Ghana</li></country></list><tree><noCountry><name sortKey="Frimpong, M" sort="Frimpong, M" uniqKey="Frimpong M" first="M" last="Frimpong">M. Frimpong</name></noCountry><country name="Ghana"><noRegion><name sortKey="Awuah, R T" sort="Awuah, R T" uniqKey="Awuah R" first="R T" last="Awuah">R T Awuah</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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